Perovskite Quantum Dots Supplier - Alfa Chemistry

Graphene Quantum Dot Fluorescent Probe for Alkaline Phosphatase Activity Detection


Graphene Quantum Dot Fluorescent Probe for Alkaline Phosphatase Activity Detection

Alfa Chemistry has always been committed to providing customers with the best quality service. With quantum dot fluorescent probes, the detection of ALP activity in human serum can be well completed. Alfa Chemistry's professional team strengthens cooperation to develop a series of high-quality solutions for customers.


ALP is a hydrolytic enzyme with specificity for phosphate substrates, which can catalyze the hydrolysis or dephosphorylation of a variety of phosphorus-containing compounds in alkaline media. In biological analysis, ALP can be used as an enzyme label or indicator in a reaction to quantify the combined process of immunoassay and genetic testing or other methods. At present, the detection methods of ALP activity mainly include colorimetric method, fluorescence analysis method, electrochemical analysis method and surface enhanced Raman scattering method. Among them, the fluorescence analysis method has greater detection advantages due to its fast, simple and sensitive signal transduction ability. Copper-phenanthroline complex, polydopamine nanoparticles, cobalt hydroxide-copper nanoclusters, silver fluorescent probes such as nanoclusters, gold nanoparticles, molybdenum disulfide quantum dots and carbon quantum dots are widely used in the detection of ALP activity. Based on the properties of benzoquinones that can statically quench the fluorescence of GQDs, a GQDs fluorescent probe can be used to efficiently detect ALP activity.


Alfa Chemistry's technical experts offer fluorescent probe designs from quantum dots and a full suite of custom alkaline phosphatase activity detection services. Our services include the following:

  • Custom of Quantum Dot Fluorescent Probes
  • Detection of Alkaline Phosphatase Activity

Highly sensitive detection of ALP

Hydrogen peroxide (H2O2) is catalyzed by horseradish peroxidase (HRP) to produce hydroxyl groups, which can oxidize o-DHB to o-benzoquinone and quench GQDs fluorescence. Ascorbic acid 2-phosphoric acid (AAP) can be catalyzed by ALP to produce ascorbic acid (AA). AA with strong reducing ability can effectively remove H2O2 and hydroxyl in solution, and then inhibit the formation of o-quinone, inducing the fluorescence recovery of GQDs. As the activity of ALP increased, the fluorescence at 440 nm increased continuously, so a highly sensitive detection method for ALP was established.

Advantages of our services

  • Years of experience and experience in customized solutions.
  • Experienced experimental technicians, exquisite experimental operation level, strict experimental control system, fast delivery cycle of data results, while ensuring that the test results are objective and reliable.
  • Complete instrument platform, high standard laboratory, higher sensitivity, more accurate detection data.
  • Competitive price.
  • Safety, all experiments were conducted under a confidentiality agreement, with emphasis on customer privacy.

Service process

The process of services includes:

  • Send us an email and Make requirements for required dimensions
  • Preparation of materials
  • Graphene quantum dot fluorescent probe for alkaline phosphatase activity detection
  • Test report and product plan are mailed back to customer

Service process

With novel technology platform and professional scientist team, Alfa Chemistry offers clients a wide range of analysis services, and we promise to provide customized solutions to meet each customer's unique requirements.

Our customer service representatives are enthusiastic and trustworthy 24 hours a day, Monday to Friday. Please do not hesitate to contact us for more information or to discuss in detail, if you are interested in our services.

* It should be noted that our service is only used for research, not for clinical use.


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