Anti-human CD4 Conjugated Cd Quantum Dots
Product Description
Alfa Chemistry can provide customers with anti-human CD4 conjugated Cd quantum dots in the emission spectral range from 525 nm to 665 nm, with excellent chemical and optical stability, good quality stability and high quantum yield. Our anti-human CD4 conjugated Cd quantum dots are available for applications in biomarkers, cell imaging, fluorescent probes, etc.
Applications
Fluorescent Labeling
The CD4 molecule is a surface marker for T helper cells and has been shown to be the primary cellular receptor for human immunodeficiency viruses HIV-1, HIV-2 and SIV. It has been shown that the spatial arrangement of CD4 on the cell membrane is important for its binding efficiency to viruses. Chen et al. combined near-field scanning optical microscopy (NSOM) and quantum dot (QDs) fluorescent labeling techniques to study the nanoscale organization of CD4 molecules at a spatial resolution of ~100 nm. The NSOM/QDs-based system directly obtained simultaneous topographic maps of T helper cells and fluorescence images of QDs. Histograms of the intensity and size distribution of QDs fluorescent dots confirmed that approximately 80% of CD4 molecules were organized in nanometer-sized domains randomly distributed on the cell surface. Intensity-size correlation analysis revealed heterogeneity in the molecular packing density of the domains. The results illustrate the combination of NSOM imaging and QDs labeling as an ultra-sensitive, high-resolution technique to probe the nanoscale organization of cell surface molecules.
Fig. 1 Combined topograph and near-field fluorescence image. (Chen et al., 2008)
Biological Applications
Optically active CdS@ZnS QDs were successfully synthesized by Zarnani et al. and used to label amine-reactive biomolecules. During the synthesis, the reaction conditions were optimized to obtain a narrow size distribution with a sharp exciton peak at 525 nm. To gain insight into the ability of the functionalized QD to bind to biomolecules, the QD was combined with an anti-human CD4 mAb. The QD surface was functionalized with organic ligands and extracted into aqueous media to allow effective binding of CdS@ZnS QD to the antibody. The QD525-bound antibody was further used to evaluate the QD functionalization method by SDS-Page. The conjugation method has the potential to be used for biological applications of QD.
Fig. 2 Topographic AFM images of cadmium selenide sample: (a) distribution network of the synthesized QD particles; (b) surface height distribution of the cadmium selenide QDs. ( Zarnani et al., 2018)
Alfa Chemistry can provide a variety of complex and customized fluorescent quantum dot products. Our products involve perovskites precursors, perovskites quantum dots, quantum dot kit, single layer quantum dots, upconverting nanoparticles and other fluorescent quantum dots. Alfa Chemistry provides products with high fluorescence quantum yield, stable quality and relatively low price. Our products are constantly updated. If the product you need is not in our catalog, please feel free to contact us, we provide relevant custom services.
References
- Chen, Jianan, et al. Nanoscale organization of CD4 molecules of human T helper cell mapped by NSOM and quantum dots[J]. Scanning: The Journal of Scanning Microscopies, 2008, 30(6): 448-451.
- Zarnani, A. H. , et al. Bioimaging Based on the Antibody-Conjugated Amphiphilic Polymer-Core@Shell Quantum Dots[J]. Emerging materials research, 2018, 7(4):1-37.
* It should be noted that our service is only used for research, not for clinical use.
