Anti-human CD8 Conjugated Cd Quantum Dots
Product Description
Alfa Chemistry can provide customers with anti-human CD8 conjugated Cd quantum dots with excellent chemical and optical stability, good mass stability, and high quantum yields. Our anti-human CD8 conjugated Cd quantum dots have emission spectra ranging from 525 nm to 665 nm and can be used for biomarkers, cell imaging, fluorescent probes, and other applications.
Features
The emission spectrum of a quantum dot can be controlled by changing the size of the quantum dot.
Quantum dots have excellent photostability.
Quantum dots have a wide excitation spectrum and a narrow emission spectrum.
Quantum dots have a large Stokes shift. Another optical property of quantum dots different from organic dyes is the wide Stokes shift, which can avoid the overlap of emission spectra and excitation spectra, and is conducive to the detection of fluorescence spectral signals.
Good biocompatibility. After various chemical modifications, quantum dots can be specifically linked, and their cytotoxicity is low, which is less harmful to living organisms and can be biologically labeled and detected in vivo.
The fluorescence lifetime of quantum dots is long. The fluorescence lifetime of organic fluorescent dyes is generally only a few nanoseconds (which is comparable to the time of spontaneous fluorescence decay of many biological samples).
Applications
Cellular Markers
The ratio of CD4+/CD8+ cells is important in the immunodiagnosis of immunodeficiency and HIV. A four-color staining scheme (DNA, CD3, CD4 and CD8) was developed for automated SFM analysis of lymphocytes. Bocsi et al. stained EDTA uncoagulated blood with different combinations of organic and inorganic (quantum dot) fluorescent stains. Aliquots were measured with flow cytometry (FCM) and SFM. Samples were scanned and digitized by SFM using four sets of fluorescent filters. Automated cellular assays, CD3, CD4 and CD8 assays were performed and CD4/CD8 ratios were calculated. Fluorescent signals were well separated on SFM and FCM. The Cusum test did not show a significant deviation from linearity (p>0.10). This comparison suggests that there is no systematic bias between the two different methods. In SFM analysis, inorganic quantum dot staining was very stable in PBS compared to organic fluorescent dyes, but bleached shortly after mounting with antioxidant and free radical scavenger mounting media. This demonstrates the difficulty of combining organic dyes and quantum dots. Slide-based multi-fluorescent labeling systems and automated SFM are suitable tools for determining the CD4/CD8 ratio in peripheral blood samples. Quantum dots are stable inorganic fluorescent labels that can be used as reliable high-resolution dyes for cell labeling.
Alfa Chemistry can provide a variety of complex and customized fluorescent quantum dot products. Our products involve perovskites precursors, perovskites quantum dots, quantum dot kit, single layer quantum dots, upconverting nanoparticles and other fluorescent quantum dots. Alfa Chemistry provides products with high fluorescence quantum yield, stable quality and relatively low price. Our products are constantly updated. If the product you need is not in our catalog, please feel free to contact us , we provide relevant custom services.
Reference
- Bocsi, J. , et al. Automated four color CD4/CD8 analysis of leukocytes by scanning fluorescence microscopy using quantum dots[J]. Cytometry Part A, 2003, 69A:131-134.
* It should be noted that our service is only used for research, not for clinical use.
