Anti-human Digoxigenin Conjugated Cd Quantum Dots
Product Description
Quantum dots are very small nanometer-sized semiconductor particles. Due to their small size, they have different optical and electronic properties compared to larger particles. Different types of quantum dots produce specific frequencies in response to electricity or light. The frequencies emitted by quantum dots can be tuned by changing the size, shape and material used for their structure. Anti-human digoxigenin conjugated Cd quantum dots with emission spectra ranging from 525 nm to 665 nm are available from. Alfa Chemistry with excellent chemical stability and optical stability, good mass stability, and high quantum yields.
Applications
Immunochromatographic Test Strips
Staphylococcus aureus is classified as the second most common foodborne pathogen, causing diseases such as pneumonia, pseudomembranous colitis, pericarditis and even sepsis. A rapid, simple and sensitive assay is needed to monitor food contamination with S. aureus. Therefore, Chen et al. developed a novel immunochromatographic test strip (ICTS) based on a two-component reaction (i.e. digoxin and anti-digoxin, quantum dot-linked streptavidin and biotin) test line with QDs as indicators for rapid and sensitive detection of S. aureus. Genomic DNA of boil-lysed bacteria was easily and rapidly extracted with silica-coated magnetic nanoparticles, and a species-specific gene was amplified by PCR using digoxigenin/biotin-labeled primers. The fluorescence of the QD tags captured on the test and control lines was observed with UV light as a signal. The sensitivity and specificity of ICTS was estimated using food samples spiked with bacteria (artificially mixed with E. coli, Listeria monocytogenes and Lactobacillus plantarum cell counts (108 CFU/mL of each bacteria)). The detection limits for S. aureus were 3 × 100 CFU/mL and 3 × 101 CFU/g in spiked milk powder and meat samples, respectively, independent of those non-Staphylococcus aureus strains.
Fig. 1 (A) Schematic illustration of the test strip and (B) detection of S. aureus using QDs/AuNPs-based ICTS. (Chen et al., 2014)
Fluorescence In Situ Hybridization
Quantum dots were proposed as a new fluorescent dye for fluorescent in situ hybridization. Quantum dot fluorescence in situ hybridization was applied to detect ebv-encoded small RNA, the most abundant viral product in latently infected cells, in paraffin-embedded tissue sections of gastric cancer. Peng et al. used quantum dot streptavidin coupling as a secondary reporter factor and digoxigenin-labeled ebv-encoded small RNA oligonucleotide probe as a detection molecule by indirect FISH. The sensitivity of quantum dot fluorescence in situ hybridization for the detection of ebv-encoded gastric cancer small RNA was slightly higher than that of chromogenic in situ hybridization.
Alfa Chemistry can provide a variety of complex and customized fluorescent quantum dot products. Our products involve perovskites precursors, perovskites quantum dots, quantum dot kit, single layer quantum dots, upconverting nanoparticles and other fluorescent quantum dots. Alfa Chemistry provides products with high fluorescence quantum yield, stable quality and relatively low price. Our products are constantly updated. If the product you need is not in our catalog, please feel free to contact us, we provide relevant custom services.
References
- Chen, Xingxing, et al. Development of a rapid and sensitive quantum dot-based immunochromatographic strip by double labeling PCR products for detection of Staphylococcus aureus in food[J]. Food Control, 2014, 46: 225-232.
- Peng, Jun, et al. Detection of epstein-barr virus infection in gastric carcinomas using quantum dot-based fluorescence in-situ hybridization[J]. Journal of Nanoscience and Nanotechnology, 2011, 11(11): 9725-9730.
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