Anti-human EpCam Conjugated Cd Quantum Dots
Product Description
Alfa Chemistry can provide customers with anti-human EpCam conjugated Cd quantum dots in the emission spectral range from 525 nm to 665 nm. Cd quantum dots are conjugated with anti-human EpCam have unique optical properties, such as narrow emission, wide absorption and optical stability, and are widely used in cancer diagnosis, biological imaging, biosensors.
Applications
Cancer Diagnosis
Circulating tumor cells (CTCs) are valuable biomarkers for monitoring the status of cancer patients and drug efficacy. However, the number of CTCs in blood is extremely small, and it remains a challenge to isolate and detect CTCs with high efficiency and sensitivity. Min et al. proposed a method for efficient capture and simple quantification of CTCs using quantum dots and magnetic beads. Anti-EpCAM antibody-conjugated quantum dots were used to target and quantify CTCs, and anti-IgG-modified magnetic beads were used to separate the quantum dot-attached CTCs. Min’s method showed a capture efficiency of about 70-80%, enabling simple quantification of the captured CTCs based on the fluorescence intensity of the quantum dots. The present method can be effectively used to capture and simply quantify CTCs for cancer diagnosis and monitoring with high efficiency.
Fig.1 Schematic representation of CTC capturing using quantum dots and magnetic beads. a) Construction of anti-EpCAM antibody-conjugated quantum dots (anti-EpCAM-QDs) as primary nanoparticles. b) Construction of anti-mouse IgG antibody-conjugated magnetic beads (anti-IgG-MBs) as secondary particles. c) Schematics for capturing the CTCs using anti-EpCAM-QDs and anti-IgG-MBs. (Min et al., 2015)
Nanoparticle Tracking Analysis
Zhang et al. compared the characteristics of exosomes from human ovarian epithelial cells (HOSEPiC) and three epithelial ovarian cancer cell lines (OVCAR3, IGROV1 and ES-2) to investigate the differences between exosomes derived from normal and malignant cells. Two well-established colloid chemistry methods, electron microscopy (EM) and dynamic light scattering (DLS), and a relatively new method, nanoparticle tracking analysis (NTA), were used to measure the size and size distribution of exosomes. The concentration of exosomes and the expression of epithelial cell adhesion molecules (EpCAM) were measured by NTA. Quantum dots were conjugated to anti-EpCAM to label exosomes and labeled exosomes were detected by NTA in fluorescence mode. Normal cell-derived exosomes were significantly larger than malignant cell-derived exosomes, and exosomes were successfully labeled using anti-EpCAM-conjugated quantum dots. Exosomes from different cell lines may differ in size and exosomes may be considered as potential diagnostic biomarkers. NTA can be considered as a useful, efficient and objective method to study different ovarian cancer exosomes and their unique properties.
Fig. 2 Levels of EpCAM expression in the four types of exosomes as detected by western blotting. (Zhang et al., 2015)
Alfa Chemistry can provide a variety of complex and customized fluorescent quantum dot products. Our products involve perovskites precursors, perovskites quantum dots, quantum dot kit, single layer quantum dots, upconverting nanoparticles and other fluorescent quantum dots. Alfa Chemistry provides products with high fluorescence quantum yield, stable quality and relatively low price. Our products are constantly updated. If the product you need is not in our catalog, please feel free to contact us, we provide relevant custom services.
References
- Hyegeun Min, Seong-Min Jo, Hak-Sung Kim. Efficient Capture and Simple Quantification of Circulating Tumor Cells Using Quantum Dots and Magnetic Beads[J]. Small, 2015, 11(21):2536-2542.
- Zhang, W. , et al. Characterization of exosomes derived from ovarian cancer cells and normal ovarian epithelial cells by nanoparticle tracking analysis[J]. Tumor Biology, 2015.
* It should be noted that our service is only used for research, not for clinical use.
