Quantum Dot Labeling
Product Description
Alfa Chemistry can provide quantum dot labeling with efficient and site-specific labeling products for customers
The types of quantum dots we offer include:
Quantum Dot Labeling Biotin and Streptavidin are available from 360 nm to 440 nm.
Quantum Dot Labeled Secondary Antibody are available from 460 nm to 500 nm. It is mainly used for detecting the presence of antibodies with good specificity and stable fluorescence properties for target immunoglobulins, which can be widely used in various proteomics studies, bioassays, fluorescent labeling of proteins, cells or tissues, biochips and biosensors. It can be used in a variety of proteomic studies, bioassays, protein, cell or tissue fluorescent labeling, biochips and biosensors to meet various research needs.
Applications
Fluorescent Probes
Sun et al. synthesized SA-QDs complexes using the active lipid method and used the same method to synthesize biotinylated FA-PAMAM using polyamidoamine (PAMAM) dendrimers as carriers. Highly expressing SKOV3 cells, while SKOV3 cells pretreated with free FA and lung adenocarcinoma A549 cells with low FR expression were used as controls to validate the specificity of this fluorescent probe for targeting SKOV3 cells. Subsequently, a fluorescent probe without PAMAM as a carrier [biotinylated FA-polyethylene glycol (PEG) combined with SA-QDs fluorescent probe] or without biotin-streptavidin amplification system (FA-PAMAM-QDs) was used as a control to verify the dual amplification effect of the fluorescent signal of the probe. In the validation experiment of the dual amplification effect, the average fluorescence intensity of SKOV3 cells labeled with biotinylated FA-PAMAM combined with SA-QDs fluorescent probe was 120.89+3.80, which was significantly higher than that of SKOV3 cells labeled with biotinylated FA-PEG combined with SA-QDs fluorescent probe of 77.50+2.43 and FA- PAMAMQDs fluorescent probe labeled SKOV3 cells of 47.81+1.35.
Fig.1 Schematic of folic acid (FA)-polyamidoamine (PAMAM)-biotin combined with streptavidin (SA)quantum dots (QDs) in diagnostic imaging of ova rian cancer SKOV3 cells in vitro. (Sun et al., 2015)
Immunoassay
Mahdi et al. reported a highly sensitive competitive immunoassay for the detection of the mycotoxin Ochratoxin (OTA) using magnetic silica nanoparticles (NPs) fluorescently labeled with rhodamine 123 (Rho123) as a signal enhancer. The method is based on the measurement of fluorescence resonance energy transfer (FRET) from CdTe quantum dots covered with anti-OTA antibodies to the dye Rho123 on the surface of NPs. The immunoreaction between the anti-OTA antibody and the OTA brings the fluorescent moiety (as an acceptor) close to the QD (as a donor), which leads to FRET upon photoexcitation of the QD. The method has a detection limit of 0.8 picograms of OTA per mL. it is applied to the determination of OTA in spiked human serum. This highly sensitive homogeneous competitive detection scheme is simple, fast and efficient.
Fig.2 Excitation light excites the immobilized QDs. (Mahdi et al., 2016)
Alfa Chemistry can provide a variety of complex and customized fluorescent quantum dot products. Our products involve perovskites precursors, perovskites quantum dots, quantum dot kit, single layer quantum dots, upconverting nanoparticles and other fluorescent quantum dots. Alfa Chemistry provides products with high fluorescence quantum yield, stable quality and relatively low price. Our products are constantly updated. If the product you need is not in our catalog, please feel free to contact us, we provide relevant custom services.
References
- Pingfeng, Sun , et al. Streptavidin-modified quantum dots combined with biotinylated folic acid in diagnostic imaging of ovarian cancer SKOV3 cells in vitro[J]. tumor, 2015.
- Mahdi, M , B. Mansour , and M. Afshin. Competitive immunoassay for Ochratoxin a based on FRET from quantum dot-labeled antibody to rhodamine-coated magnetic silica nanoparticles[J]. Microchimica Acta, 2016.
* It should be noted that our service is only used for research, not for clinical use.
