Water Soluble Fluorescent Quantum Dots
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Product Description
Fluorescent quantum dots have the unique advantages of high fluorescence quantum yield, adjustable fluorescence emission spectrum, good biocompatibility and stable optical properties, with narrow and symmetric emission bands. Alfa Chemistry can provide quantum dots with wavelengths ranging from 525 nm to 605 nm, and can also customize products according to customer requirements.
Applications
Biosensors
Speckman et al. developed a multi-pathogen fiber optic detector based on immunoassay using fluorescent semiconductor quantum dots (QDs) of different sizes as reporting labels in order to facilitate overcoming these problems. In order to develop such a quantum dot-based biosensor, it was necessary to demonstrate that the quantum dots could be attached to antibody proteins, thereby maintaining antibody specificity. Speckman et al. have been working to develop a reproducible method for attaching QDs to antibodies for bioassay applications. First, fluorescent QDs of different sizes were synthesized, their surfaces were functionalized with several types of organic groups to obtain water solubility, and these functionalized QDs were covalently attached to rabbit anti-albumin antibody proteins. It was demonstrated by testing that these labeled antibodies exhibited selective binding to ovalbumin antigen, and UV-Vis absorption spectroscopy and picosecond (psec) transient photoluminescence (TPL) spectroscopy were also performed to characterize the QDs at each step throughout the synthesis process. TPL spectroscopy measurements showed that the QD lifetimes depended on the size of the QDs, the intensity of the photoexcitation source, and whether they were functionalized and attached to the antibody linkage.
Fluorescent Probes
Diagnostic detection of influenza viruses can be performed using fluorescent quantum dots, which are a promising emerging fluorescent probe that can play an important role in detecting pathogens. 2012, Wen et al. prepared a quantum dot-conjugated secondary antibody by covalently linking fluorescent quantum dots to a rabbit anti-chicken IgY antibody. The conjugate was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorescence spectrophotometry. And then immunosorbent assay with fluorescent quantum dots was performed to determine the concentration of influenza B virus. It was found that the rabbit anti-chicken IgY antibody increased its molecular weight when coupled with fluorescent quantum dots, but the emission spectrum did not change. More importantly, the luminescence intensity of the quantum dots showed a linear relationship with the virus concentration. These results may provide a potential new method for quantifying viruses, pathogenic bacteria and toxic molecules.
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References
- Speckman, D. M., et al. Biodetection using fluorescent quantum dots. Technologies, Systems, and Architectures for Transnational Defense, 2002.
- Wen, J. L., et al. Blue Fluorescent Quantum Dots-Based Probe for Detection of Influenza B Virus. Advanced Materials Research, 2012, 365:18-22.
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