Water-phase CdTe/CdS/ZnS (carboxyl) Quantum Dots
Product Description
Alfa Chemistry provides water-phase CdTe/CdS/ZnS (carboxyl) quantum dot products are CdTe as the core, CdSe and ZnS as the shell layer, the surface is wrapped by carboxyl ligands core/shell/shell type fluorescent nanomaterials. Storage should avoid direct sunlight, 4 degrees sealed dark storage, can be customized for customers to produce 540-780 nm any wavelength of different grams of products. The product has the characteristics of uniform particle size, broad absorption spectrum, symmetric emission spectrum, high and stable fluorescence intensity.
Applications
Biological Applications
In the work of Ulusoy et al., three-dimensional (3D) spheroid cultures of human adipose-derived mesenchymal stem cells (hAD-MSCs) with tissue-mimicking morphology through well-developed cell-cell and cell-matrix interactions and unique diffusion/transport properties were evaluated for dose-dependent toxicity of red-emitting CdTe/CdS/ZnS quantum dots (Qdots) effects. Morphological investigations and time-resolved microscopy analysis as well as cellular metabolic activity studies showed that 3D spheroid cultures were more resistant to Qdot-induced cytotoxicity compared to conventional 2D cultures. The results obtained suggest the presence of two different cell populations in 2D cultures with different sensitivity to Qdot, however no such effect was observed in 3D spheroids. The study by Ulusoy et al. aims to improve the prediction of Qdot nanotoxicity at the tissue level and provide the necessary screening step before any in vivo application. Furthermore, the ability of highly fluorescent Qdot to penetrate densely packed spheroids will enhance the biological application of the developed Qdot in tissue structures.
Fig. 1 Fluorescence imaging of hAD-MSCs spheroids treated with CdTe/CdS/ZnS Qdots for 4 h. (A) Fluorescence images captured from fluorescence microscopy from left-to-right: bright-field, Qdot (red), Calcein-AM (green) and DAPI (blue). (B) Single plane confocal images collected with 10× objective, from left-to-right: bright-field, DAPI (blue), Qdot (red), merge image of Qdot + DAPI and magnified spheroid. (C) Z-stacked confocal images of spheroid at individual z-planes. (D) Single-plane confocal fluorescence images of spheroid at the bottom surface. (Ulusoy et al., 2015)
Cell Imaging
Tian et al. reported the use of CdTe/CdS/ZnS core-shell shell QDs for imaging hepatocellular carcinoma cells and for immunoassay of alpha-fetoprotein (AFP). Stable and high PLQY (20%-48%) CdTe/CdS/ZnS core-shell-shell QDs were synthesized by a stepwise process. Bioconjugation of core-shell-shell QDs with streptavidin (SA) was successfully applied to immunofluorescence imaging of human hepatocellular carcinoma (HCC) cell line HepG2.2.15. In addition, thioglycolic acid (TGA)-capped CdTe/CdS/ZnS core-shell QDs have a longer fluorescence lifetime than fluorescein and were therefore engaged for the first time in conjugation with antigen for immunoassay of protein (AFP) by fluorescence polarization.
Fig. 2 Schematic illustration of aqueous synthesis of CdTe/CdS/ZnS QDs and bioconjugation (a); immunofluorescent labeling of cancer cells (b) and the reaction between the antibody (Ab) and the free antigen (Ag) and labeled (QDs-Ag) antigen (c). (Tian et al., 2010)
Alfa Chemistry can provide a variety of complex and customized fluorescent quantum dot products. Our products involve a variety of fluorescent quantum dot products. Alfa Chemistry provides products with high fluorescence quantum yield, stable quality and relatively low price. Our products are constantly updated. If the product you need is not in our catalog, please feel free to contact us, we provide relevant custom services.
References
- Ulusoy, M. , et al. Evaluation of CdTe/CdS/ZnS core/shell/shell quantum dot toxicity on three-dimensional spheroid cultures[J]. Toxicology Research, 2015, 5.
- Tian, Jianniao, et al. Synthesis of CdTe/CdS/ZnS quantum dots and their application in imaging of hepatocellular carcinoma cells and immunoassay for alpha fetoprotein[J]. Nanotechnology, 2010, 21(30): 305101.
* It should be noted that our service is only used for research, not for clinical use.
